Development and Application of an RT-PCR to Differentiate the Prevalent NA-PRRSV Strains in China
Yanlin Li1, 2, #, Guobiao Ji2, #, Xiaodong Xu1, 2, Juan Wang2, Yingying Li2, Feifei Tan2, Xiangdong Li2, *
Identifiers and Pagination:Year: 2017
Issue: Suppl-1, M4
First Page: 66
Last Page: 72
Publisher Id: TOVJ-11-66
Article History:Received Date: 06/10/2016
Revision Received Date: 01/11/2016
Acceptance Date: 06/02/2017
Electronic publication date: 30/06/2017
Collection year: 2017
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PRRSV features with genetic diversity and high mutation which leads to the emergence of a multiple of circulating virus strains with different virulence. North American (genotype 2) PRRSV (NA-PRRSV) can be divided into classical PRRSV (C-PRRSV), highly pathogenic PRRSV (HP-PRRSV), and NADC30-like PRRSV (NL-PRRSV) according to their genomic characteristics and pathogenicity. So far, the above three subtypes of NA-PRRSV are now circulating in China.
Objective and Method:
In this study, a reverse transcript polymerase chain reaction (RT-PCR) was established to simultaneously differentiate three subtypes of NA-PRRSV. The established RT-PCR can be applied to PRRSV-infected samples originated from both supernatant of cell culture and pig tissues and showed specificity exclusively to PRRSV. The sensitivity of RT-PCR showed the minimum RNA detection was 0.04ng/µl.
Result and Conclusion:
The established RT-PCR was next used to differentiate the subtypes of 29 NA-PRRSV isolated in 2016 and the results showed that HP-PRRSV is still the dominant circulating virus strain in the presence of NADC30-like PRRSV in Henan province.