Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China

Zhi Zhou, Jiajun Wu, Shuo Zhang, Bo Hou, Tao Han, Jing Wang, Qi Xu, Dongyue Wang, Yinyi Liu, Shengpeng Xin, Xinyan Zhai*
China Animal Disease Control Center, OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, No. 20 Maizidian Street, Chaoyang District, Beijing 100125, China.

© 2017 zhou et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the China Animal Disease Control Center, No. 20 Maizidian Street, Chaoyang District, Beijing, 100125, P. R. China; Tel: +86-10-59198898; Fax: +86-10-59198899;



NADC30-like strains of porcine reproductive and respiratory syndrome virus first appeared in Chinese swine herds in 2012.

Objective and Method:

To explore the possible genetic diversity of these strains, we sequenced and analyzed the complete genomes of two NADC30-like strains. These isolates shared 95.4% homology with NADC30.


The two strains displayed a discontinuous deletion of 131 amino acids in NSP2, mutations of amino acids in GP3 and GP5, and a 3-nucleotide deletion in the 3′ untranslated region. Phylogenetic analysis showed that the two isolates formed a new branch and clustered in a subgroup with NADC30 isolates from North America.


We conclude that the above two NADC30-like strains may have been introduced from North America to China, where they acquired new genetic diversity.

Keywords: PPRSV, NADC30-like strain, Genetic diversity, Virus.