Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation
Amit Kr Chakraborty1, 2, Priyanka Mukherjee1, 2, Amarjit Karam1, Samir Das1, Luit Barkalita3, Kekungo Puro1, Rajkumari Sanjukta1, Sandeep Ghatak1, Ingudam Sakuntala1, Ram Gopal Laha1, Prabodh Borah3, S.V. Ngachan1, Indu Sharma2, Arnab Sen1, *
Identifiers and Pagination:Year: 2018
Issue: Suppl-2, M7
First Page: 110
Last Page: 120
Publisher Id: TOVJ-12-110
Article History:Received Date: 16/4/2017
Revision Received Date: 6/7/2018
Acceptance Date: 16/7/2018
Electronic publication date: 31/08/2018
Collection year: 2018
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.
Methods and Material:
The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5’ UTR.
Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5’ UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.