RESEARCH ARTICLE


Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation



Amit Kr Chakraborty1, 2, Priyanka Mukherjee1, 2, Amarjit Karam1, Samir Das1, Luit Barkalita3, Kekungo Puro1, Rajkumari Sanjukta1, Sandeep Ghatak1, Ingudam Sakuntala1, Ram Gopal Laha1, Prabodh Borah3, S.V. Ngachan1, Indu Sharma2, Arnab Sen1, *
1 Division of Animal Health, ICAR Research Complex for NEH, Barapani - 793103, India
2 Department of Microbiology, Assam University, Silchar - 788011, India
3 Department of Biotechnology, C.V.Sc, AAU, Khanapara, Assam, India


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 1126
Abstract HTML Views: 626
PDF Downloads: 293
ePub Downloads: 259
Total Views/Downloads: 2304
Unique Statistics:

Full-Text HTML Views: 705
Abstract HTML Views: 368
PDF Downloads: 176
ePub Downloads: 143
Total Views/Downloads: 1392



© 2018 Chakraborty et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Division of Animal Health, ICAR Research Complex for NEH, Barapani - 793103, India; E-mail: arnabsen123@gmail.com


Abstract

Introduction:

The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.

Methods and Material:

The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5’ UTR.

Results:

Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5’ UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.

Keyowords: Bovine viral diarrhea virus, Porcine, North east, Sequence analysis, Phylogenetic analysis, India.