RESEARCH ARTICLE


Molecular Characterization of BK Polyomavirus’ Large T Antigen Gene Sequences Detected in Prostate Cancer Tissues of Sudanese Patients



Babbiker M. T. Gorish1, *, Mohammed E. H. Ournasseir1, Iman M. Shammat1, 2
1 Department of Microbiology and Immunology, Faculty of Medical Laboratory Science - Omdurman Islamic University, Omdurman, Sudan
2 Department of Biology, Faculty of Science, Taibah University, Medina – Yanbu Branch, Saudi Arabia


© 2019 Gorish et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Microbiology and Immunology, Faculty of Medical Laboratory Science, Omdurman Islamic University, Omdurman, Sudan; E-mail: qorish456@gmail.com


Abstract

Background:

BK virus, which is associated with Prostate Cancer (PCa), have a global seroprevalence in humans. Based on the sequences of VP1 and the Large Antigen (LTAg) genes, there are four subtypes of BKV. Each subtype has its own subgroups.

Objective:

The aim of this study was to identify the BKV subtype that circulates among Sudanese patients with PCa.

Materials and Methods:

A total of 8 samples from our previous work on BKV were studied in this investigation. The LTAg gene was partially amplified (176nt) by a homemade PCR. All the amplicons were purified and subjected to sequencing. Bioedit version 7.0 and Mega X version 6.0 were used to analyze the sequence and compare the results with the BKV sequences and build a phylogenetic tree.

Results:

All the BKV LTAg gene sequences derived from Sudanese patients were classified with Subtype-1 BKV strains from Iran and Japan. Translated protein alignment showed that some isolates had identical amino acids with Iranian and Japanese strains, whereas others had a silent mutation. Interestingly, a point mutation was identified in the sequences of isolate 5 and 8 where adenine nucleotide (A) was replaced with Cytosine (C) at position 276, resulting in amino acid substitution.

Conclusion:

It was concluded that all the BKV isolates which circulated among Sudanese prostate tumor patients belonged to subtype 1. These findings only highlighted the need for the molecular detection and subtyping of BKV strains in Sudanese patients in order to better demonstrate the relationship between BKV infection and PCa.

Keywords: BK virus, Large T Antigen, PCR, Genotype, Prostate, Cancer, Benign prostatic hyperplasia, Point mutation.