Phylogenesis and Clinical Aspects of Pandemic 2009 Influenza A (H1N1) Virus Infection



Massimo Ciccozzi 1, Muhammed Babakir-Mina 2, 3, Alessandra Lo Presti 1, Fabbio Marcuccilli 2, Carlo Federico Perno 2, 3, Marco Ciotti*, 2
1 Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita’, Rome, Italy
2 Laboratory of Molecular Virology, Foundation Polyclinic Tor Vergata, Viale Oxford, 81- 00133, Rome, Italy
3 Virology, Department of Experimental Medicine and Biochemistry, Tor Vergata University, Rome, Italy


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© Ciccozzi et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Laboratory of Molecular Virology, Foundation Polyclinic Tor Vergata, Viale Oxford, 81-00133, Rome, Italy; Tel.: +39 06 20902087; Fax: +39 06 20902078; E-mail: marco.ciotti@ptvonline.it


Abstract

During the spring of 2009, a new influenza A (H1N1) virus of swine origin emerged and spread worldwide causing a pandemic influenza. Here, 329 naso-pharyngeal swabs collected from patients with flu-like symptoms were analyzed by real-time PCR for the presence of H1N1 2009 pandemic virus. Twenty-five samples collected from immunocompetent and immunodepressed patients contained the H1N1 pandemic virus. Phylogenetic analysis of the hemagglutinin and neuraminidase genes showed no obvious differences in terms of similarity and/or homology between the sequences identified in immunocompetent individuals and those obtained from immunocompromised patients. Pre-existing clinical conditions may influence the outcome of H1N1 disease.

Keywords: H1N1 pandemic virus, swine flu, respiratory infections, phylogenetic analysis, influenza surface antigens..