Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology



Kazunari Kondo 1, Asami Uenoyama 1, Ryo Kitagawa 1, Hajime Tsunoda 1, Rika Kusumoto-Matsuo 2, Seiichiro Mori 2, Yoshiyuki Ishii 2, Takamasa Takeuchi 2, Tadahito Kanda 3, Iwao Kukimoto*, 2
1 NTT Medical Center Tokyo, 5-9-22 Higashi-gotanda, Shinagawa-ku, Tokyo 141-8625, Japan
2 WHO HPV LabNet Regional Reference Laboratory, WHO Western Pacific Region, Pathogen Genomics Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-murayama, Tokyo 208-0011, Japan
3 Center of Research Network for Infectious Diseases, Riken, 1-101 Kanda-jinbocho, Chiyoda-ku, Tokyo 101-0051, Japan


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© Kondo et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Pathogen Genomics Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan; Tel: +81 42 561 0771; Fax: +81 42 567 5632; E-mail: ikuki@nih.go.jp


Abstract

We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen with minimum bias. Total DNA was extracted from cervical exfoliated cells collected from 326 outpatients with abnormal Pap smears. Overall, 307 specimens (94%) were HPV-positive, 30% of which contained multiple genotypes. The prevalence of HPV DNA was 83% (49/59 samples) in atypical squamous cells of undetermined significance (ASC-US); 91% (20/22 samples) in atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H); 97% (130/134 samples) in low-grade squamous intraepithelial lesion (LSIL); and 99% (85/86 samples) in high-grade squamous intraepithelial lesion (HSIL). Three most frequent HPV types detected in HSIL were HPV16 (36%), HPV52 (24%), and HPV58 (14%). Our results suggest that multiple HPV infections are more prevalent in Japanese women than previously reported, and confirm that HPV52 and 58 are more dominant in their cervical precancerous lesions when compared to those reported in Western countries.

Keywords: Human papillomavirus, HPV genotyping, cervical cancer, Pap smear, abnormal cytology, HPV vaccine..