Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Dengue Capsid
Suganya Selvarajah 1, Udayan Chatterji 1, Richard Kuhn 2, Richard Kinney 3, Subhash G Vasudevan 4, Philippe Gallay*, 1
Identifiers and Pagination:Year: 2012
First Page: 29
Last Page: 37
Publisher Id: TOVJ-6-29
Article History:Received Date: 23/11/2011
Revision Received Date: 20/1/2012
Acceptance Date: 20/1/2012
Electronic publication date: 29/3/2012
Collection year: 2012
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.