Selvarajah , Suganya; Chatterji , Udayan; Kuhn , Richard; Kinney , Richard; Vasudevan , Subhash G; Gallay, Philippe

Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Dengue Capsid

Suganya Selvarajah ¹, Udayan Chatterji ¹, Richard Kuhn ², Richard Kinney ³, Subhash G Vasudevan ⁴, Philippe Gallay^{*, 1}

¹ Department of Immunology & Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA

² Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA

³ Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522, USA

⁴ Program in Emerging Infectious Diseases, DUKE-NUS Graduate Medical School, Singapore

Article Information

Identifiers and Pagination:

Year: 2012
Volume: 6
First Page: 29
Last Page: 37
Publisher Id: TOVJ-6-29
DOI: 10.2174/1874357901206010029

Article History:

Received Date: 23/11/2011
Revision Received Date: 20/1/2012
Acceptance Date: 20/1/2012
Electronic publication date: 29/3/2012
Collection year: 2012

© Selvarajah et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

^* Address correspondence to this author at the Department of Immunology an& Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA; Tel: 858-784-8180; Fax: 858-784-8831; E-mail: gallay@scripps.edu

The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.

Keywords: Capsid, Dengue, ELISA, IFSA..

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RESEARCH ARTICLE

Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Dengue Capsid

Article Information

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