The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
Jianbao Dong1, 2, 3, Wei Zhu1, Akatsuki Saito1, Yoshitaka Goto1, Hiroyuki Iwata4, Takeshi Haga*, 1
Identifiers and Pagination:Year: 2012
First Page: 68
Last Page: 72
Publisher Id: TOVJ-6-68
Article History:Received Date: 27/1/2012
Revision Received Date: 14/3/2012
Acceptance Date: 29/3/2012
Electronic publication date: 8/6/2012
Collection year: 2012
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Matrix protein is known to have an important role in the process of virus assembly and virion release during measles virus replication. In the present in vitro study, a single mutation of E89K in the matrix protein was shown to affect cell death and virus replication efficiency in human PBMC. One strain with this mutation caused less cell death than the parental virus, and possessed high virus replication efficiency. Moreover, by Annexin V-FITC staining, polycaspase FLICA staining, and double labeling with poly-caspase FLICA and the Hoechst stain, the cell death seen was shown to be apoptosis.