Tropism Analysis of Two Coxsackie B5 Strains Reveals Virus Growth in Human Primary Pancreatic Islets but not in Exocrine Cell Clusters In Vitro

Hodik M* , Lukinius A, Korsgren O, Frisk G
Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden

Article Metrics

CrossRef Citations:
Total Statistics:

Full-Text HTML Views: 163
Abstract HTML Views: 198
PDF Downloads: 58
Total Views/Downloads: 419
Unique Statistics:

Full-Text HTML Views: 112
Abstract HTML Views: 68
PDF Downloads: 44
Total Views/Downloads: 224

© Hodik et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Rudbecklaboratoriet, Dag Hammarskjöldsväg 20, 751 85 Uppsala, Sweden; Tel: +46735720471; Fax: +46 (18) 611 02 22; E-mail:


Human Enteroviruses (HEVs) have been implicated in human pancreatic diseases such as pancreatitis and type 1 diabetes (T1D). Human studies are sparse or inconclusive and our aim was to investigate the tropism of two strains of Coxsackie B virus 5 (CBV-5) in vitro to primary human pancreatic cells. Virus replication was measured with TCID50 titrations of aliquots of the culture medium at different time points post inoculation. The presence of virus particles or virus proteins within the pancreatic cells was studied with immunohistochemistry (IHC) and electron microscopy (EM). None of the strains replicated in the human exocrine cell clusters, in contrast, both strains replicated in the endocrine islets of Langerhans. Virus particles were found exclusively in the endocrine cells, often in close association with insulin granules. In conclusion, CBV-5 can replicate in human endocrine cells but not in human exocrine cells, thus they might not be the cause of pancreatitis in humans. The association of virus with insulin granules might reflect the use of these as replication scaffolds.

Keywords: Coxsackie B virus, human enterovirus, human enterovirus capsid protein 1, human islets of langerhans, pancreas, pancreatitis, type 1 diabetes..