Detection and Genotyping of Human Papillomavirus DNA in Formalin-Fixed Paraffin-Embedded Specimens with the HPV Direct Flow CHIP System



Elsa Herraez-Hernandez1, Ovidiu Preda1, Sonia Alonso2, Rosario Serrano Pardo3 , Asuncion Olmo*, 1
1 R&D Department, Master Diagnóstica, Avenida del Conocimiento 100, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, Spain
2 Pathology Department, Elda General Hospital. Carretera Elda-Sax, La Torreta s/n, 03600, Elda, Alicante, Spain
3 Pathology Department, Ruber Clinic, Calle de Juan Bravo, 49, 28006, Madrid, Spain


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© Herraez-Hernandez, et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the R&D Department, Master Diagnóstica, Avenida del Conocimiento 100, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, Spain; Tel: +34 958 271 449; Fax: +34 958 271 434; E-mail: asuncion.olmo@vitroweb.com


Abstract

The novel HPV Direct Flow CHIP commercial system for Human Papillomavirus (HPV) genotyping is based on rapid PCR and automatic reverse dot blot hybridization to genotype-specific probes, allowing the detection of 36 HPV genotypes. This study examined the performance of HPV Direct Flow CHIP in formalin-fixed paraffin-embedded (FFPE) samples (n= 99). Each sample was analyzed both by Direct PCR, using crude cell extracts without DNA purification, and by conventional PCR, using purified DNA. Pair-wise analysis of the results demonstrated strong concordance between the results obtained with the two protocols, although a slightly higher rate of multiple infections was detected by conventional PCR. In summary, HPV Direct Flow CHIP achieves effective HPV detection from FFPE samples with both Direct PCR and Conventional PCR protocols.

Keywords: : Biopsy, cervical cancer, direct PCR, FFPE, HPV, HPV direct flow CHIP..