Molecular Detection of Human Cytomegalovirus (HCMV) Among Infants with Congenital Anomalies in Khartoum State, Sudan
Maha G. Ebrahim1, Aisha S. Ali2, Mohamed O. Mustafa3, Dalal F. Musa4, Abdel Rahim M. El Hussein3, Isam M. Elkhidir5, Khalid A. Enan3, *
Identifiers and Pagination:Year: 2015
First Page: 38
Last Page: 41
Publisher Id: TOVJ-9-38
Article History:Received Date: 12/5/2015
Revision Received Date: 16/10/2015
Acceptance Date: 20/10/2015
Electronic publication date: 10/12/2015
Collection year: 2015
open-access license: This is an open access article licensed under the terms of the (https://creativecommons.org/licenses/by/4.0/legalcode), which permits unrestricted, noncommercial use, distribution and reproduction in any medium, provided the work is properly cited.
Human Cytomegalovirus (HCMV) infection still represents the most common potentially serious viral complication in humans and is a major cause of congenital anomalies in infants. This study is aimed to detect HCMV in infants with congenital anomalies.
Study subjects consisted of infants born with neural tube defect, hydrocephalus and microcephaly.
Fifty serum specimens (20 males, 30 females) were collected from different hospitals in Khartoum State. The sera were investigated for cytomegalovirus specific immunoglobin M (IgM) antibodies using enzyme-linked immunosorbent assay (ELISA), and for Cytomegalovirus DNA using polymerase chain reaction (PCR).
Out of the 50 sera tested, one patient’s (2%) sample showed HCMV IgM, but with no detectable DNA, other 4(8.2 %) sera were positive for HCMV DNA but with no detectable IgM.
Various diagnostic techniques should be considered to evaluate HCMV disease and routine screening for HCMV should be introduced for pregnant women in this setting. It is vital to initiate further research work with many samples from different area to assess prevalence and characterize HCMV and evaluate its maternal health implications.